New VIP paper published in Angewandte Chemie
In collaboration with Prof. Marc Vendrell, we've recently published our work on fluorogenic amino acids in Angewandte Chemie. The publication, selected as a VIP paper, describes the synthesis of a new class of amino acids that are tagged with small fluorophores, which can be used easily in solid state peptide synthesis. Furthermore, the fluorogenic amino acids are dim in aqueous solution, but switch-on when in hydrophobic environments, therefore making them ideal for PAINT super-resolution microscopy. We demonstrated this feature by performing peptide-PAINT of post-synaptic densities using a PDZ-binding peptide, and showed the same nanoclusters identified by Matthew Broadhead and Mathew Horrocks (http://dx.doi.org/10.1038/srep24626) in 2016.
In the ESMB, the synthesis/imaging work was performed by Zuzanna Konieczna (funded by EASTBio), and the brain samples were prepared by Katie Morris and Takeshi Kaizuka.
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